NAIST Graduate School of Materials Science

Bio-Process Engineering

Staff & Contact
Educational StaffProf. Youichiro Hosokawa
Assistant Prof. Takeo Katayama
Assistant Prof. Ryohei Yasukuni
Assistant Prof.Takanori Iino
TEL: +81-743-72-6199

The Laser Nano-Manipulation Science Laboratory takes advantage of laser light to develop microscopic technology for the manipulation and measurement of cells and proteins, and to clarify bio-mechanical interaction between cells or proteins from a new perspective in order to further understand responsiveness of cells and living tissue by environment stress. These activities aim to open new fields of life innovation and green innovation. The laboratory fosters human resources who have a broad knowledge of science ranging from physics and chemistry to biology and medicine.

1. 1. Exploration of the responsiveness of cells and living tissues to the environment stress
2. Development of new measurement method to estimate internal stress in living tissue utilizing ultrashort laser and atomic force microscope
3. Development of new manipulation technique to stimulate and to select single cells utilizing ultrashort pulse laser
4. Kinetics of local explosion in water induced by ultrashort laser pulse and its interaction to biological micro-objects

  • Fig.1 Manipulation of micro-beads by femtosecond laser impulse
  • Fig. 2 Nanometer scale vibration of Zebrafish embryo induced by femtosecond laser impulse and detected by Atomic force microscope
  • Fig. 3 Activation of cultured biological cells by femtosecond laser impulse

1: Iino, T., Hagiyama,
M., Furuno, T., Ito, A. and Hosokawa Y.. Time-course statistical evaluation of intercellular adhesion maturation by single-cell adhesion measurement using femtosecond laser impulse. Biophysical Journal , Vol. 111, Issue 10, pp. 2255-2262 (2016).

2: Oikawa, K., Matsunaga, S., Mano, S., Kondo, M., Yamada, K., Hayashi, M., Kagawa, T., Kadota, A., Sakamoto, W., Higashi, S., Watanabe, M., Mitsui, T., Shigemasa, A., Iino, T., Hosokawa, Y. and Nishimura, M.. Physical interaction between peroxisomes and chloroplasts elucidated by in situ laser analysis. Nature Plants 1, 15035 (2015).

3. Katsuno, H., Toriyama, M., Hosokawa, Y., Mizuno, K., Ikeda, K., Sakumura, Y. and Inagaki N., Actin migration driven by directional assembly and disassembly of membrane anchored actin filaments, Cell Reports, Vol. 12, Issue 4, pp. 648-660 (2015).

4: Makenaka M., Iino, T., Nagatani, A. and Hosokawa, Y., Nanoscale bending movement of biological micro-object induced by femtosecond laser impulse and its detection by AFM, Appl. Phys. Express, Vol. 7, No. 8, pp. 087002_1-4 (2014).

5: Hirashima, T., Hosokawa, Y., Iino, T. and Nagayama, M., On fundamental cellular processes for emergence of collective epithelial movement, Biology Open, Vol. 2, No. 7, pp.660-666 (2013).

6: Hosokawa, Y., Ohta, M., Ito, A. and Takaoka, Y., Photomechanical ablation of biological tissue induced by focused femtosecond laser and its application for acupuncture, Appl. Phys. A, Vol. 110, No. 3, pp.613-616 (2013).

7: Iino, T. and Hosokawa Y., Controllability of femtosecond laser-induced impulse in water evaluated by local force measurement system using atomic force microscopy, J. Appl. Phys. , Vol.112, No. 6, pp. 066106_1-066106_3 (2012).

8: Hosokawa, Y., Hagiyama, M., Iino, T., Murakami, Y. and Ito, A., Noncontact estimation of intercellular breaking force using a femtosecond laser impulse quantified by atomic force microscopy, Proceedings of the National Academy of Sciences, Vol.108, 1777-1782 (2011).

9: Hosokawa, Y., Ochi, H., Iino, T., Hiraoka, A. and Tanaka, M., Photoporation of biomolecules into single cells in living vertebrate embryos induced by a femtosecond laser amplifier, PloS one, Vol.6, e27677 (2011).

10: Iino, T. and Hosokawa, Y., Direct measurement of femtosecond laser impulse in water by atomic force microscopy, Applied Physics Express, Vol. 3, pp. 107002 (2010).


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