Division of Materials Science, NAIST

Bio-Process Engineering Laboratory

Staff & Contact
Educational StaffProf. Yoichiroh Hosokawa
Associate Prof.Yallikun Yaxiaer
Assistant Prof. Ryohei Yasukuni, Sohei Yamada
TEL: +81-743-72-6199

The Bio-process Engineering Laboratory promotes developmental research on high-precision and fast manipulation methodologies for small biological materials, in which ultra-short pulse laser technology is utilized. When an intense femtosecond laser is focused in the vicinity of a micro-sized biological micro-object in a water medium, an explosion of water is induced at the laser focal point, and shock and stress waves due to the explosion are acted as an impulsive force on the sample (Fig. 1). We have developed several kinds of methodologies to manipulate single animal and plant cells utilizing this impulsive force. In addition, this laser manipulation technology has been combined with Atomic Force Microscope (AFM) and micro-fluidic chip technologies. The AFM is applied to quantify the impulsive force and to analyze the sample oscillation induced by the impulsive force (Fig. 2). A micro-fluidic chip is used to realize high-speed cell manipulation. For instance, these methodologies are applied to clarify biomechanical interaction between cells or proteins from a new perspective in order to further understand responsiveness of cells and living tissue under environment stress (Fig. 3). These activities aim to open new fields of life innovation and green innovation. The laboratory fosters human resources who have a broad knowledge of engineering and science from areas ranging from physics and chemistry to biology and medicine.

1.   Kinetics of local explosions in water induced by ultrashort laser pulses, and its interaction with biological micro-objects

2.  Development of new measurement methods to estimate internal stress in living tissues utilizing ultrashort lasers and atomic force microscopes

3.   Development of new cell manipulation techniques in micro-fluidic chips

4.   Exploration of the responsiveness of cells and living tissues to the environment stress and its application to cell manipulation

  • Fig.1 Manipulation of micro-beads by femtosecond laser impulse
  • Fig. 2 Nanometer scale vibration of Zebrafish embryo induced by laser impulse and detected by AFM
  • Fig. 3 High-speed laser manipulation in micro-fluidic chips.

Fig.4 Laser scanning photoporation of fluoresce probe molecules at single cell resolution

1.T. Maeno, T. Uzawa, I. Kono, K. Okano, T. Iino, K. Fukita, Y. Oshikawa, T. Ogawa, O. Iwata, T. Ito, K. Suzuki, K. Goda, Y. Hosokawa, “Targeted delivery of fluorogenic peptide aptamers into live microalgae by femtosecond laser photoporation at single-cell resolution,” Sci. Rep., 2018, 8, 8271.
2.Y. Yalikun, Y. Hosokawa, T. Iino, and Y. Tanaka, “An all-glass 12 μm ultra-thin and flexible micro-fluidic chip fabricated by femtosecond laser processing,” Lab Chip, 2016,16, 2427–2433.
3.K. Oikawa, S. Matsunaga, S. Mano, M. Kondo, K. Yamada, M. Hayashi, T. Kagawa, A. Kadota, W. Sakamoto, S. Higashi, M. Watanabe, T. Mitsui, A. Shigemasa, T. Iino, Y. Hosokawa, M. Nishimura, “Physical interaction between peroxisomes and chloroplasts elucidated by in situ laser analysis,” Nature Plants, 2015, 1, 15035.
4.Y. Hosokawa, H. Ochi, T. Iino, A. Hiraoka, M. Tanaka, “Photoporation of biomolecules into single cells in living vertebrate embryos induced by a femtosecond laser amplifier,” PLoS ONE, 2011, 6, e27677.
5.Y. Hosokawa, M. Hagiyama, T. Iino, Y. Murakami, A. Ito, “Noncontact estimation of intercellular breaking force using a femto-second laser impulse quantified by atomic force microscopy,” Proc. Nat’l Acad. Sci. USA, 2011, 108, 1777-1782.


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