Division of Materials Science, NAIST

Mr. Mark Christian Guinto, 2nd year master course student of the Photonic Device Science Laboratory, received a Neuro2019 Domestic Travel Award from the Japan Neuroscience Society and the Japanese Society for Neurochemistry.

Mr. Mark Christian Guinto of the Photonic Device Science Laboratory was given a Domestic Travel Award at Neuro2019 (42nd Annual Meeting of the Japanese Neuroscience Society and the 62nd Annual Meeting of the Japanese Society for Neurochemistry). The award supports accommodation during the meeting. Neuro2019 was held in Toki Messe, Niigata, Japan on July 25-28, 2019.

– Travel Award recipients list:
https://neuro2019.jnss.org/en/travel_award_ja.html#tae01

Investigation of neural activity related to feeding behavior in GCaMP6 transgenic mice using an implantable imaging device

Mark Christian Guinto, Yasumi Ohta, Mamiko Kawahara, Makito Haruta, Kiyotaka Sasagawa, Jun Ohta

I am deeply grateful to Professor Jun Ohta for his mentorship and for the opportunity to work on this exciting interdisciplinary research. I would also like to express my heartfelt thanks to Dr. Y. Ohta, Ms. Kawahara, Prof. Haruta and Prof. Sasagawa for lending their expertise and for their guidance in realizing this work. What I presented at Neuro2019 was indeed a fruit of collaborative effort. Finally, I really appreciate Ms. Fukuzawa for her constant support, and every member of our laboratory for maintaining an encouraging research environment.

Feeding behavior is regulated by neural circuits that are known to be involved in the processing of satiety, reward and pleasure. At present, available methods for calcium imaging in deep brain areas are still a challenge to execute without interfering with the normal behavior of an awake mouse. Our current approach centers on an implantable micro-imaging device that can visualize neural activity from calcium dynamics in the deep brain developed in our laboratory. The micro-imaging device consists of a CMOS-based image sensor chip embedded on a flexible substrate and a μ-LED that serves as an excitation light source. Deep brain implantation of the device in GCaMP6 transgenic mice demonstrated successful detection of neuronal activity in regions related to feeding behavior, including the lateral and the arcuate nucleus of the hypothalamus, sections of the striatum, and the amygdala. Distinct patterns of somatic fluorescence were recorded from the sampled regions over a range of behaviors (e.g., grooming, eating, resting) exhibited by the animal. Taken together, our ultra-light, compact device presents a useful paradigm for exploring neural dynamics in freely moving setups, cueing potential for simultaneous imaging of multiple sites across neural circuits.

 

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