NAIST 奈良先端科学技術大学院大学 物質創成科学領域 ~光ナノサイエンス~

光機能素子科学研究室のMark Christian Guintoさん(博士前期課程2年)がNEURO2019(第42回日本神経科学大会・第62回日本神経化学会大会)にて国内Travel Awardを獲得

2019年7月25~28日に新潟 朱鷺メッセにて開催されたNEURO2019(第42回日本神経科学大会・第62回日本神経化学会大会)で、光機能素子科学研究室のMark Christian Guintoさん(博士前期課程2年)が国内Travel Awardを獲得しました。本賞は227名の応募に対し50名の学生に贈られました。

Investigation of neural activity related to feeding behavior in GCaMP6 transgenic mice using an implantable imaging device

Mark Christian Guinto, Yasumi Ohta, Mamiko Kawahara, Makito Haruta, Kiyotaka Sasagawa, Jun Ohta

I am deeply grateful to Professor Jun Ohta for his mentorship and for the opportunity to work on this exciting interdisciplinary research. I would also like to express my heartfelt thanks to Dr. Y. Ohta, Ms. Kawahara, Prof. Haruta and Prof. Sasagawa for lending their expertise and for their guidance in realizing this work. What I presented at Neuro2019 was indeed a fruit of collaborative effort. Finally, I really appreciate Ms. Fukuzawa for her constant support, and every member of our laboratory for maintaining an encouraging research environment.

Feeding behavior is regulated by neural circuits that are known to be involved in the processing of satiety, reward and pleasure. At present, available methods for calcium imaging in deep brain areas are still a challenge to execute without interfering with the normal behavior of an awake mouse. Our current approach centers on an implantable micro-imaging device that can visualize neural activity from calcium dynamics in the deep brain developed in our laboratory. The micro-imaging device consists of a CMOS-based image sensor chip embedded on a flexible substrate and a μ-LED that serves as an excitation light source. Deep brain implantation of the device in GCaMP6 transgenic mice demonstrated successful detection of neuronal activity in regions related to feeding behavior, including the lateral and the arcuate nucleus of the hypothalamus, sections of the striatum, and the amygdala. Distinct patterns of somatic fluorescence were recorded from the sampled regions over a range of behaviors (e.g., grooming, eating, resting) exhibited by the animal. Taken together, our ultra-light, compact device presents a useful paradigm for exploring neural dynamics in freely moving setups, cueing potential for simultaneous imaging of multiple sites across neural circuits.

 

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