Quantification of adhesion strength between sub-organelles inside plant cell
The quantification technique of intercellular adhesion between animal cells was applied to evaluation between chloroplasts and peroxisomes inside plant cell. Peroxisome is a sub-organelle to help photorespiration when chloroplast performs photosynthesis. Physiological studies of the interactions between chloroplasts and peroxisomes during photorespiration found that these organelles actively communicated under photosynthetic conditions through dynamic change of their morphology. In this mechanism, adhesion control between chloroplasts and peroxisomes is entail key factor. We applied our method to the analysis and succeeded in estimating the light-depending interaction with Brownian motion.
Left microphotographs: peroxisomes (green) and chloroplasts (grey) before and after the laser shooting. Asterisks indicate laser focal point.
Right histograms: the probability of a peroxisome detaching from a chloroplast.
Kazusato Oikawa, Shigeru Matsunaga, Shoji Mano, Maki Kondo, Kenji Yamada, Makoto Hayashi, Takatoshi Kagawa, Akeo Kadota, Wataru Sakamoto, Shoichi Higashi, Masakatsu Watanabe, Toshiaki Mitsui, Akinori Shigemasa, Takanori Iino, Yoichiroh Hosokawa, Mikio Nishimura, "Physical interaction between peroxisomes and chloroplasts elucidated by in situ laser analysis", Nature Plants, vol.Vol. 1, Article number: 15035.(2015)"Back"