The Bio-processing Engineering Laboratory organized by Prof. Hosokawa promotes developmental research on high-precision and fast manipulation methodologies for biological materials, such as cells and proteins, in which state-of-the-art laser technology is combined with microscopic manipulation and detection technologies. Especially we have the world’s top facilities and activities on cell manipulation and processing by femtosecond laser amplifier and have attracted attention as a pioneer of laser applications for biotechnology.
Research（Jump to past research）
High throughput cell sorting system
We develop a high throughput cell sorting system in a microfluidic chamber based on the impulsive force induced by a focused femtosecond laser in water.
Ultra-thin glass processing method
We have developed an ultra-thin glass processing method without thermal shock stress using an ultra-short pulse laser technology.
Quantitatively evaluating the adhesive strength
We aim to establish a method for quantitatively evaluating the adhesive strength from the detachment dynamics of adherent cells by applying femtosecond laser impulse.
Physiological responses of cells to the mechanical stimuli
We are developing a spatiotemporal control technique of cellular functions through the understanding of physiological responses of cells to the mechanical stimuli induced by the femtosecond laser.
Molecule introduction into cells using femtosecond laser
We are developing a method to introduce various molecules into a single cell and control its function in a tissue using femtosecond laser.Past research（Jump to present research）
Quantification of impulse
Quantification femtosecond laser-induced impulsive force.Appl.Phys.Express. 3:107002(2010).
Adhesion between animal cells
Noncontact estimation of intercellular adhesion strengthProc. Natl. Acad. Sci. USA 108(2011).
Adhesion in plant cell
Quantification of adhesion strength between sub-organelles inside plant cellNat. Plants 1, 15035 (2015).
Photoporation
Delivery of biomolecules into targeted single cells in living vertebrate embryosPLoS ONE Vol. 6, No. 11(2011).
Stiffness of biological tissue
Precise evaluation of stiffness of micro-sized biological objectAppl. Phys. Express 7, 087002 (2014).
