about Bio-Process Engineering Laboratory

The Bio-processing Engineering Laboratory organized by Prof. Hosokawa promotes developmental research on high-precision and fast manipulation methodologies for biological materials, such as cells and proteins, in which state-of-the-art laser technology is combined with microscopic manipulation and detection technologies. Especially we have the world’s top facilities and activities on cell manipulation and processing by femtosecond laser amplifier and have attracted attention as a pioneer of laser applications for biotechnology.

High throughput cell sorting system

We develop a high throughput cell sorting system in a microfluidic chamber based on the impulsive force induced by a focused femtosecond laser in water.

Ultra-thin glass processing method

We have developed an ultra-thin glass processing method without thermal shock stress using an ultra-short pulse laser technology.

Quantitatively evaluating the adhesive strength

We aim to establish a method for quantitatively evaluating the adhesive strength from the detachment dynamics of adherent cells by applying femtosecond laser impulse.

Physiological responses of cells to the mechanical stimuli

We are developing a spatiotemporal control technique of cellular functions through the understanding of physiological responses of cells to the mechanical stimuli induced by the femtosecond laser.

Molecule introduction into cells using femtosecond laser

We are developing a method to introduce various molecules into a single cell and control its function in a tissue using femtosecond laser.

Past research(Jump to present research

Quantification of impulse

Quantification femtosecond laser-induced impulsive force.
Appl.Phys.Express. 3:107002(2010).

Adhesion between animal cells

Noncontact estimation of intercellular adhesion strength
Proc. Natl. Acad. Sci. USA 108(2011).

Adhesion in plant cell

Quantification of adhesion strength between sub-organelles inside plant cell
Nat. Plants 1, 15035 (2015).


Delivery of biomolecules into targeted single cells in living vertebrate embryos
PLoS ONE Vol. 6, No. 11(2011).

Stiffness of biological tissue

Precise evaluation of stiffness of micro-sized biological object
Appl. Phys. Express 7, 087002 (2014).

Ice formation

Spatial and temporal control of ice formation under microscope