Adhesion between animal cells

Noncontact estimation of intercellular adhesion strength

Adhesion between cells is strongly linked with essential biological functions such as structuring of tissue, infiltration of cancer cells, and information propagation between cells. However, there is no method to evaluate intercellular adhesion with variety conformation. We established a methodology to evaluate the adhesion strength between cells utilizing the femtosecond laser-induced impulsive force. We realized to quantify the adhesion strength without mechanical contact to the cell and tissue, which can see in microscope.

<Figure 1>
<Figure 1>Femtosecond laser-induced intercellular dissociation of leukocyte attached to endothelial cell monolayer. Red dots in right images indicate laser focal point.

<Figure 2>
<Figure 2>Femtosecond laser-induced intercellular dissociation of MDCK cell-cell interface. Red points indicate laser focal point.

with Prof. Akihiko Ito (Kinki Univ.)

Yoichiroh Hosokawa, Mitsuru Hagiyama, Takanori Iino, Yoshinori Murakami, Akihiko Ito, "Non-contact Estimation of Intercellular Breaking Force Using Femtosecond Laser Impulse Quantified by Atomic Force Microscopy," Proc. Natl. Acad. Sci., vol.108, pp1777-1782.(2011)